Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues.
Identifieur interne : 001223 ( Main/Exploration ); précédent : 001222; suivant : 001224Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues.
Auteurs : Sacha Escamez [Suède] ; Benjamin Bollhöner [Suède] ; Hannele Tuominen [Suède]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- cytologie : Arabidopsis, Xylème.
- métabolisme : Arabidopsis, Xylème.
- méthodes : Histocytochimie.
- Microscopie de fluorescence, Mort cellulaire, Survie cellulaire, Transport biologique.
English descriptors
- KwdEn :
- MESH :
- cytology : Arabidopsis, Xylem.
- metabolism : Arabidopsis, Xylem.
- methods : Histocytochemistry.
- Biological Transport, Cell Death, Cell Survival, Microscopy, Fluorescence.
Abstract
Histochemical assays of xylem cell death cannot take advantage of the conventional methods for detection of cell death, such as staining with propidium iodide or trypan/Evans blue or the TUNEL staining. This chapter presents two alternative histochemical methods that can be used to detect xylem cell death quickly and reliably using light microscopy. The first method is a viability stain that can be used to detect cell death of different types of xylem elements in basically any plant species. The second method reveals cell death in xylem vessel elements based on their functionality in transport of water and small water-soluble stains.
DOI: 10.1007/978-1-4939-6722-3_3
PubMed: 28050826
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<front><div type="abstract" xml:lang="en">Histochemical assays of xylem cell death cannot take advantage of the conventional methods for detection of cell death, such as staining with propidium iodide or trypan/Evans blue or the TUNEL staining. This chapter presents two alternative histochemical methods that can be used to detect xylem cell death quickly and reliably using light microscopy. The first method is a viability stain that can be used to detect cell death of different types of xylem elements in basically any plant species. The second method reveals cell death in xylem vessel elements based on their functionality in transport of water and small water-soluble stains.</div>
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